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You should defend with hands that are profitable for you to defend based on your skill level, so I wouldn't jump to 65% right away.
For a 3bb open you should, at a minimum, be defending the following from the BB vs the BU with 100bb stacks:
22+ A9o+ A2s+ KTo+ K8s+ QTo+ Q9s+ JTo+ J8s+ T9s T8s 98s 97s 87s 76s 65s
This is 25% of hands and should get you started. You can add to this once you get more comfortable with postflop play, and if you're uncomfortable flatting with some of those hands, you can try 3-betting them instead. The small suited connectors are best for this.
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