It all comes down to your range of hands, his range of hands and the board. Mostly the problem is if you are doing this on weak scared hands (betting 1/4 pot) what are you doing with your best hands. You need to blur the line between our lower end of our range and our higher end. Second pair still has equity vs certain hands on certain boards. If you are talking AQ on a Q high flop and a K turn is far different from JTs on a T high flop and a K turn. The question is very vague for details of this type. Why not find a hand, give us some stats or a read and start from there.

Betting 1/4 pot on the turn will almost never be right. Giving guys with second pair, GS draws and even an Ax bottom pair a redraw with so much money behind is never good. Biggest problem between limit and 100BB no limit is the money behind becomes more important than the pot.