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Well then he probably isn't making a play is he? I'd make it 34 and fold. With 100bb stacks just shove.

If you think he's making a play then just 5bet pre. You have a nice hand for that play.

Also please don't reveal results.

16bb 4bet and we're oop against nit with AQo slightly deep sure looks like a fold to me without very significant skill gap

I don't think the river is a very good card to bluff on as it doesn't affect ranges very much at all. I really hate the call pre.